Network pharmacology, bioinformatics, molecular docking and experimental verification of the mechanism of HXZTS treatment on osteoarthritis synovium

Chen, L.; Wang, H.; Wu, D.; Su, J.; Chen, S.; Chen, D.; Zhang, T.; He, W.

doi:10.1101/2025.09.25.678423

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bioRxiv · 10.1101/2025.09.25.678423

Network pharmacology, bioinformatics, molecular docking and experimental verification of the mechanism of HXZTS treatment on osteoarthritis synovium

Chen, L.·Wang, H.·Wu, D.·Su, J.·Chen, S.·Chen, D.·Zhang, T.·He, W.

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Abstract

BackgroundOsteoarthritis (OA) is a prevalent joint disease characterized by synovial inflammation and synovial fibroblast dysfunction. Elucidating the molecular mechanisms underlying synovial tissue impairment in OA is crucial for developing targeted therapeutic interventions. Huoxuezhitongsan (HXZTS), a traditional Chinese medicine formula, has shown promising therapeutic effects in treating OA, but its mechanisms of action on synovial tissue remain unclear. MethodThis study integrated network pharmacology, bioinformatics analysis, molecular docking, and experimental validation to elucidate the potential targets, pathways, and mechanisms by which HXZTS exert therapeutic effects on osteoarthritic synovial tissue. ResultsNetwork analysis identified 21 active ingredients and 111 common targets of HXZTS for OA, with key compounds like quercetin, {beta}-sitosterol, and stigmasterol. Enrichment analysis revealed HXZTS modulates inflammation through TNF, IL-17, and MAPK signaling pathways, oxidative stress, apoptosis, angiogenesis, and metabolic regulation. Molecular docking demonstrated strong binding affinities of HXZTS compounds with targets like AKT1, TNF, TP53, IL6, and VEGFA. RT-qPCR validation confirmed the downregulation of inflammatory mediators like IL6 and PTGS2, alongside the upregulation of genes associated with potential cartilage repair effects, such as MMP1 and MMP3, upon HXZTS treatment. ConclusionsHXZTS exerts therapeutic effects on osteoarthritic synovial tissue through a multifaceted mechanism involving modulation of inflammation, oxidative stress, apoptosis, angiogenesis, and metabolic regulation. These findings provide insights into the potential application of HXZTS as a complementary or alternative therapeutic approach for managing OA and a foundation for developing novel targeted therapies.

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Chen, L., Wang, H., Wu, D., Su, J., Chen, S., Chen, D., Zhang, T., He, W.. 2025-09-29. Network pharmacology, bioinformatics, molecular docking and experimental verification of the mechanism of HXZTS treatment on osteoarthritis synovium. https://doi.org/10.1101/2025.09.25.678423

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