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bioRxiv · 10.1101/2025.09.22.675323

Inhibition of 3-mercaptopyruvate sulfurtransferase enhances CD8⁺ T-cell antitumor immunity

Abstract

Hydrogen sulfide (H2S) is a redox-active gasotransmitter implicated in tumor progression and immune regulation. The enzyme 3-mercaptopyruvate sulfurtransferase (3-MST) is a key contributor to endogenous H2S and polysulfide production, but its role in tumor-immune interactions remains poorly defined. Here, we show that 3-MST is the most abundantly expressed H2S-synthesizing enzyme in human renal cell carcinoma cells (RCC) and that high 3-MST expression correlates with reduced patient survival. Pharmacological inhibition of 3-MST lowered intracellular H2S levels in Renca renal carcinoma cells, suppressed proliferation, induced apoptosis, and increased surface expression of the immunogenic markers CD70, CD86, and PD-L1. In immune cells, partial inhibition of 3-MST promoted T cell activation, as evidenced by increased CD69 expression on both CD4 helper and CD8 cytotoxic T cells. In contrast, complete inhibition of 3-MST, achieved by high concentrations of the inhibitor, modestly reduced CD8 T cell proliferation. Functionally, 3-MST inhibition potentiated antigen-specific CD8 T cell-mediated killing of tumor cells, an effect further amplified by PD-L1 blockade. These results establish 3-MST as a redox-sensitive metabolic driver of tumor growth and immune evasion in RCC and demonstrate that its inhibition can boost antitumor immune responses, offering a potential avenue for combination immunotherapy.

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BibTeXRIS

Urwyler, M., Korsos, M., Dupuychaffray, E., Ascencao, K., Petrosino, M., Zuhra, K., Tachet, J., Purwar, P., Alvarez, M., Pommier, A., Gad, M., Abdelkader, R., Szabo, C., Bourquin, C.. 2025-09-23. Inhibition of 3-mercaptopyruvate sulfurtransferase enhances CD8⁺ T-cell antitumor immunity. https://doi.org/10.1101/2025.09.22.675323

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