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bioRxiv · 10.1101/2025.09.11.675682

Weak SLP-76-PLC-γ1 interaction in the LAT-nucleated multi-protein complex fine-tunes TCR signal strength to optimize T cell responsiveness

Abstract

Upon TCR engagement several protein tyrosine kinases are recruited and activated, and adapter proteins and enzymes are phosphorylated on tyrosine residues, leading to further events characterizing activated T cells. Phosphorylation of the LAT adapter protein enables binding of the enzyme PLC-{gamma}1 and of a dimer of two additional adapter proteins Gads and SLP-76, forming a tetrameric structure. Within this heterotetramer there is a weak interaction between SLP-76 and PLC-{gamma}1, and the relevant binding sites of SLP-76 and PLC-{gamma}1 are highly conserved in vertebrates. To address the biological relevance of this weak interaction, we introduced a mutation in the SLP-76 that enhanced its affinity for PLC-{gamma}1 and found that this mutation increased PLC-{gamma}1 activity and altered thymocyte development and peripheral T cell responses due to enhanced TCR signal strength. The conserved weak SLP-76-PLC-{gamma}1 interaction is critical for the controlled activation of PLC-{gamma}1, thus fine-tuning TCR signal strength to optimize T cell-mediated immunity.

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BibTeXRIS

Yamane, H., Wada, J., Stassenko, E. N., Convertino, N. D., Lee, M. E., Vacchio, M. S., Li, W., Rathnayake, U. M., Balagopalan, L., Chari, R., Hagenau, H., Awasthi, P., McGavern, D., Bosselut, R., Samelson, L. E.. 2025-09-17. Weak SLP-76-PLC-γ1 interaction in the LAT-nucleated multi-protein complex fine-tunes TCR signal strength to optimize T cell responsiveness. https://doi.org/10.1101/2025.09.11.675682

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