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bioRxiv · 10.1101/2025.09.11.675326

Antimycin A, but not antimycin A3 or myxothiazol, directly suppresses photosystem II activity

Imaizumi, K.·Ifuku, K.
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Abstract

Antimycin A (AA) is a widely used inhibitor to study photosynthesis and respiration. In photosynthesis, it is commonly used to inhibit a cyclic electron flow (CEF) pathway but has also been reported to affect photosystem II (PSII), which is not involved in CEF. Although concerns have been raised about AAs specificity, its impact on PSII activity remains unclear. AA3 was recently proposed as a more specific inhibitor of the same CEF pathway. In the mitochondrial respiratory chain, AA inhibits complex III, similar to myxothiazol. Here, we investigated the direct effects of AA, AA3, and myxothiazol on PSII activity and linear photosynthetic electron transport using isolated plant PSII and thylakoid membranes. AA, but neither AA3 nor myxothiazol, directly suppressed PSII activity and linear electron transport. Furthermore, the extent of AAs effects was batch-dependent. Thus, we propose using AA3 to inhibit CEF and myxothiazol to inhibit complex III, instead of AA. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=158 SRC="FIGDIR/small/675326v1_ufig1.gif" ALT="Figure 1"> View larger version (21K): org.highwire.dtl.DTLVardef@a61461org.highwire.dtl.DTLVardef@1841ac5org.highwire.dtl.DTLVardef@1f9a345org.highwire.dtl.DTLVardef@4e155c_HPS_FORMAT_FIGEXP M_FIG C_FIG Antimycin A inhibits at least three sites. Antimycin A3 has higher specificity for CEF-PSI, while myxothiazol is preferred to inhibit complex III.

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Imaizumi, K., Ifuku, K.. 2025-09-16. Antimycin A, but not antimycin A3 or myxothiazol, directly suppresses photosystem II activity. https://doi.org/10.1101/2025.09.11.675326

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