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bioRxiv · 10.1101/2025.09.05.674552

Peripheral inflammation mediates midbrain Lrrk2 kinase activity via Rab32 expression

Abstract

Mutations that increase leucine-rich repeat kinase 2 (LRRK2) activity confer significant risk for Parkinsons disease (PD), yet incomplete disease penetrance suggest additional factors are required to manifest disease. We recently identified RAB32 Ser71Arg as a Mendelian gene for PD. Here, we establish Rab32 as a key mediator linking peripheral inflammation to Lrrk2 activation. We show that Rab32 and Rab38 expression are modestly, but inversely, correlated with their homolog Rab29. In vivo, peripheral lipopolysaccharide (LPS)-induced inflammation selectively induced Rab32 expression in midbrain Iba1+ microglia but not dopaminergic neurons, where it localized to Lamp1+ lysosomal compartments and correlated with Lrrk2 kinase activity. LPS similarly induces Rab32 expression in human induced pluripotent stem cell-derived microglia, demonstrating a unified biological response to inflammation across species. Promoter analysis identified Tfe3, a master regulator of lysosomal biogenesis and autophagy, as a key driver of Rab32 expression induced Lrrk2 kinase activation. During inflammation, Tfe3 translocated to the nucleus of midbrain Iba1+ microglia to induce Rab32 expression and Lrrk2 kinase activity. Knockdown of Tfe3, but not Tfeb, mitigates these effects, establishing Rab32 as a physiological rheostat of Lrrk2 activity. This mechanistic pathway enables peripheral inflammation to modulate LRRK2 activity and highlights Rab32/Tfe3 as a therapeutic targeting for neuroprotection in PD.

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BibTeXRIS

Follett, J., Deng, I. B., Sharp, R. C., Wall, S. C., Mamais, A., Farrer, M. J.. 2025-09-06. Peripheral inflammation mediates midbrain Lrrk2 kinase activity via Rab32 expression. https://doi.org/10.1101/2025.09.05.674552

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