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bioRxiv · 10.1101/2025.09.05.674255

Enhancers mediate euchromatin hopping at chromatin contact points

Abstract

Enhancer-mediated gene activation involves the recruitment of chromatin modifiers and RNA polymerase to target promoters, but it is unknown if enhancers influence chromatin beyond their target genes. Euchromatin and heterochromatin associated histone modifications separate the genome into opposing nuclear compartments. Whereas heterochromatin marks are known to spread from one modified nucleosome to another, no such ability has been ascribed to euchromatin. Using mono-allelic enhancer deletions, native ChIP-seq, and an engineered interaction between an enhancer and transcriptionally inert DNA, we show that enhancers mediate the acquisition of euchromatin features at distal regions through chromatin looping. We term this phenomenon euchromatin hopping and found it occurring on average [~]270kb bidirectionally from enhancers, redefining our understanding of enhancer-mediated chromatin architecture with implications on enhancer identification using chromatin features. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=129 SRC="FIGDIR/small/674255v2_ufig1.gif" ALT="Figure 1"> View larger version (33K): org.highwire.dtl.DTLVardef@12c6e9aorg.highwire.dtl.DTLVardef@1165feorg.highwire.dtl.DTLVardef@3f08c8org.highwire.dtl.DTLVardef@598bd3_HPS_FORMAT_FIGEXP M_FIG C_FIG Euchromatin hopping modelFigure showing the proposed euchromatin hopping model. TFs recognize and bind to their binding sites in an active enhancer region. Upon activation enhancers recruit coactivators and RNAPII forming a condensate that supports gene activation. After an abundance of transcriptional machinery and coactivators are recruited, adjacent TF bound sites acquire euchromatin features through physical proximity to the active compartment, we call these regions "bystanders". Upon enhancer deletion, condensate formation is lost and active euchromatin marks are not acquired at the gene promoter or other enhancer chromatin contacts. TFs are displayed in yellow, coactivators in green, RNAPII in pink, and histone modifications in red.

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BibTeXRIS

Mitchell, J. A., Mullany, S., Taylor, T., Li, H., Zhao, Y., Suri, A., Sexton, T., Davies, J. O.. 2025-09-05. Enhancers mediate euchromatin hopping at chromatin contact points. https://doi.org/10.1101/2025.09.05.674255

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