Altered excitability of dI3 neurons regulates hindlimb motor tone and locomotor recovery after spinal cord injury

Abstract

Recovery of motor function after spinal cord injury is limited in mammals. Reactivation of locomotor circuits does occur, but primarily through the activation of sensorimotor pathways in the context of locomotor training. Previous investigations have shown that dI3 neurons, a developmentally-defined population of pre-motor, glutamatergic interneurons, are indispensable for this process. However, it remains unclear how dI3 neurons are recruited during locomotor recovery, and whether they could be leveraged to improve locomotor function following spinal cord injury. Herein, we investigated how the excitability of dI3 neurons influences locomotor behaviour and recovery after spinal cord injury. In T9-T10 transected mice, we found that acute chemogenetic silencing of dI3 neurons leads to immediate loss of hindlimb motor tone, and significant reduction in stepping during treadmill locomotion. Conversely, regular chemogenetic stimulation of dI3 neurons led to transient increases in hindlimb motor tone early after injury, but ultimately reduced hindlimb motor tone and locomotor recovery over the long term. These chronic changes resulting from dI3 neuron stimulation were associated with the absence of expression of the constitutive 5-HT2C-R isoform, potentially representing a homeostatic mechanism for the regulation of dI3 excitability following spinal cord injury. Given these findings, we hypothesized that dI3 stimulations effects on motor tone, while insufficient to drive locomotor function alone, may promote stepping improvements when locomotor rhythm-generating circuits are active. The addition of quipazine, a serotonergic agonist known to facilitate locomotor rhythmogenesis, in combination with dI3 stimulation, significantly improved locomotor function, while also mitigating the long-term reduction in treadmill stepping associated with dI3 stimulation alone. In aggregate, our results suggest that hyper-excitable dI3 neurons are involved in the maintenance of motor tone after spinal cord injury, possibly through a 5-HT2C-R-dependent mechanism, and further show that the selective stimulation of dI3 neurons could enhance the recovery of locomotor function following spinal cord injury.