NGN2 Expression and Regional Patterning Allow Rapid Differentiation from hiPSCs to DRG-Like Neurons Responsive to Type 2 Cytokines

Habich, C.; Miller, L. N.; Granillo Luz, N.; Iqbal Malik, L.; Chegireddy, K.; Maneshi, M. M.; Wolf, M.; Kummer, M.; Iriki, H.; Roewe, J.; Marro, S.; Scott, V. E.; Smith, K. M.; Kim, B.; Reinhardt, P.; Goedken, E. R.

doi:10.1101/2025.08.27.672624

bioRxiv · 10.1101/2025.08.27.672624

NGN2 Expression and Regional Patterning Allow Rapid Differentiation from hiPSCs to DRG-Like Neurons Responsive to Type 2 Cytokines

Habich, C.·Miller, L. N.·Granillo Luz, N.·Iqbal Malik, L.·Chegireddy, K.·Maneshi, M. M.·Wolf, M.·Kummer, M.·Iriki, H.·Roewe, J.·Marro, S.·Scott, V. E.·Smith, K. M.·Kim, B.·Reinhardt, P.·Goedken, E. R.

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Abstract

Itch or pruritus, is a sensation that elicits scratching behaviour and is a major symptom and cause of morbidity in skin diseases such as atopic dermatitis (AD), allergic contact dermatitis (ACD), prurigo nodularis (PN), and urticaria. Itch is often triggered by inflammatory stimuli in the skin including type 2 cytokines such as IL-4, IL-13, and/or IL-31. Several therapies targeting type 2 immune pathways have been developed to treat pruritus; however, itch improvement in many patients remains to be improved. Thus, additional approaches to modulate sensory neuron activity are needed. Ex vivo or even in vitro study of the molecular mechanisms underlying primary sensory neuron activation is challenging since harvesting neurons from dorsal root ganglia (DRG) in patients can only be done from cadavers. Herein, we describe rapid human sensory neurons generation (2 days to precursor cells) by in vitro differentiation of human induced pluripotent stem cells (hiPSC) from simultaneous application of patterning factors with NGN2 overexpression. We show that these hiPSC-derived sensory neurons possess key characteristics of primary sensory neurons. They express key neuronal markers, such as TRKA receptors, TRPV1 and TRPA1 channels, and functionally respond to the TRPV1 agonist capsaicin. In addition, they express key type 2 cytokine receptors such as interleukin (IL)-4R and IL31-R, known to promote itch in AD and PN. Moreover, these cells are functional as our sensory neurons respond to IL-4, IL-13 and IL-31 stimulation. Collectively, these data demonstrate that our protocol generates a phenotypic profile consistent with native somatosensory neurons that can facilitate development of novel approaches to model and treat pruritic disease.

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Habich, C., Miller, L. N., Granillo Luz, N., Iqbal Malik, L., Chegireddy, K., Maneshi, M. M., Wolf, M., Kummer, M., Iriki, H., Roewe, J., Marro, S., Scott, V. E., Smith, K. M., Kim, B., Reinhardt, P., Goedken, E. R.. 2025-09-01. NGN2 Expression and Regional Patterning Allow Rapid Differentiation from hiPSCs to DRG-Like Neurons Responsive to Type 2 Cytokines. https://doi.org/10.1101/2025.08.27.672624

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