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bioRxiv · 10.1101/2025.08.25.672195

Lipid composition determines thermotropic properties of meibum of animals and humans with Meibomian gland disorders

Butovich, I. A.·Wojtowicz, J. C.·Wilkerson, A.·Yuksel, S.
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Abstract

Meibum - a lipid-rich secretion produced by holocrine Meibomian glands (MG) - plays a central role in maintaining ocular surface homeostasis. Previously, changes in MG lipidomes induced by inactivation of critical genes of meibogenesis, such as Elovl3, Soat1, Awat2, and others, were shown to cause MG-dysfunction- and dry eye-like signs in mice. Here, we describe the impact of the lipid composition of meibum on its physiological properties, specifically thermotropic/melting characteristics, using various wild type and mutant animals, and compare them with meibum of healthy humans and patients with abnormal meibum. Meibum samples were analyzed using liquid chromatography/mass spectrometry (LC/MS) and differential scanning microcalorimetry (DSC). We found that changes in the balance between major lipid classes in meibum - wax esters, cholesteryl esters, triacylglycerols and free cholesterol - cause detrimental changes in its properties, leading to the loss of cohesiveness, abnormal expressibility from MG, a decline in its protective functions, and, ultimately, abnormal phenotypes of the eyes and adnexa. We conclude that tested animal species can be valuable models for studying human diseases. A combination of LC/MS and DSC can be a powerful diagnostic tool and may help to determine the molecular reasons of various pathologies in human subjects and animals.

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Butovich, I. A., Wojtowicz, J. C., Wilkerson, A., Yuksel, S.. 2025-08-29. Lipid composition determines thermotropic properties of meibum of animals and humans with Meibomian gland disorders. https://doi.org/10.1101/2025.08.25.672195

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