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bioRxiv · 10.1101/2025.08.23.671898

Engineering non-exponential proliferation in Escherichia coli using functionalized protein aggregates

Van Eyken, R.·Jordens, S.·Oome, D.·Broux, K.·Aertsen, A.
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Abstract

Uncurbed exponential proliferation might not always be required for genetically modified microorganisms, and might even cause unpredictable liabilities in their behavior and impact. We therefore constructed a bacterial chassis that adheres to linear proliferation for a finite number of generations, based on the asymmetric segregation of a gradually declining intracellular protein aggregate that is engineered to produce a conditionally essential metabolite such as cAMP.

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Van Eyken, R., Jordens, S., Oome, D., Broux, K., Aertsen, A.. 2025-08-23. Engineering non-exponential proliferation in Escherichia coli using functionalized protein aggregates. https://doi.org/10.1101/2025.08.23.671898

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