Early-life adversity modulates growth trajectories and red blood cell mitochondrial metabolism in king penguin chicks

Abstract

In many avian species, variation in breeding phenology is known to affect reproductive success. In king penguins, the breeding cycle lasts more than a year, and the start of egg-laying extends over 3 months, with early-breeders laying eggs around December and late-breeders around February. Consequently, late-born chicks have less time to grow and build up their energy reserves before the Austral winter. It is however not known whether late-born chicks display alternative physiological strategies to catch up before winter. Variation in metabolic rate is one important pathway driving differences in growth patterns, as it is directly involved both in energy allocation processes and fitness. The conversion of resources into energy occurs in mitochondria, and the efficiency of this conversion is likely to play a fundamental role in explaining individual heterogeneity in growth and survival. The purposes of this study were to investigate the differences in red blood cell mitochondrial metabolism between early- and late-born king penguin chicks and to assess whether morphometric phenotypes could be explained by differences in mitochondrial metabolism. Late-chicks expressed higher mitochondrial metabolism compared to early-chicks at 100 days post-hatching, probably linked to the stress related to winter environmental conditions and a decrease in parental feeding rates. We did not find a clear association between chick mitochondrial metabolism and growth patterns, suggesting that mostly environmental conditions contributed in explaining different metabolic phenotypes. As king penguin populations in Crozet may face changing breeding conditions (changes in feeding area and foraging trips duration) in relation to global changes, studying the physiological traits and adaptations underlying the chick growth and survival may help understanding the response of king penguins facing challenging conditions.