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bioRxiv · 10.1101/2025.08.17.670750

An open, integrated platform for multiplexed bioluminescence microscopy

Scipioni, L.·Torrado, B.·Tedeschi, G.·Halbers, L.·Torrey, Z. R.·Fuller, E. B.·Fersini, F.·Gohlke, C.·Luptak, A.·Prescher, J. A.·Digman, M. A.
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Abstract

We report an imaging package to democratize all-in-one bioluminescence and fluorescence microscopy. The platform comprises three tools: PhasorViewer, a visualization suite to design experiments and identify optimal probe combinations; PhasorScope, an open-source, cost-effective microscopy framework to upgrade conventional microscopes; and PhasorAnalysis, a dedicated, user-friendly analysis pipeline to quantify phasor imaging data. We demonstrate the utility of the platform for multiplexed, simultaneous fluorescence and bioluminescence imaging with readily accessible optical reporters.

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Scipioni, L., Torrado, B., Tedeschi, G., Halbers, L., Torrey, Z. R., Fuller, E. B., Fersini, F., Gohlke, C., Luptak, A., Prescher, J. A., Digman, M. A.. 2025-08-22. An open, integrated platform for multiplexed bioluminescence microscopy. https://doi.org/10.1101/2025.08.17.670750

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