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bioRxiv · 10.1101/2025.08.15.670588

m6ASeqTools: An R toolkit for post-processing m6A sites detected by m6Anet

Balaj, L.·Lee, H.·Gashi, D.·Batool, S. M.·Escobedo, A. K.·Carter, B. S.
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Abstract

m6ASeqTools is an R package designed to streamline the post-processing and interpretation of site-level m6A predictions from m6Anet. It provides descriptive summaries of m6A distribution across transcripts, genes, biotypes, and transcript regions, and enables condition comparisons through a calculated weighted modification ratio. By integrating differential gene expression data, the package links methylation changes with expression differences, providing biotype-specific and region-specific insights into how m6A localization patterns relate to transcriptional regulation. Availability and implementationm6ASeqTools is freely available at https://github.com/hannalee809/m6ASeqTools Supplementary informationSupplementary data are available at Bioinformatics online.

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Balaj, L., Lee, H., Gashi, D., Batool, S. M., Escobedo, A. K., Carter, B. S.. 2025-08-21. m6ASeqTools: An R toolkit for post-processing m6A sites detected by m6Anet. https://doi.org/10.1101/2025.08.15.670588

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