High throughput in situ imaging reveals complex ecological behaviour of giant marine mixotrophic protists

Abstract

Mixotrophic organisms have the ability to combine photosynthetic and heterotrophic nutrition, and are ubiquitous in marine ecosystems. Here, we focus on Rhizaria, a diverse group of planktonic protists that included both mixotrophic and non-mixotrophic taxa, and that are particularly delicate and poorly sampled by standard plankton nets. Using high-frequency in situ imaging, we study the ecology of these organisms directly in their undisturbed environment. We examined the fine-scale distribution and orientation of ~230,000 organisms belonging to three groups of Rhizaria, including the mixotrophic taxa Acantharia and Collodaria, and the non-mixotrophic Phaeodaria. Overall, our results suggest that mixotrophic protists have better ability to control their position within the water column (depth, orientation) than non-mixotrophic ones. Our observations also point to several steps in the obscure life cycle of the mixotrophic Collodaria, during which de novo symbiont acquisition appears to involve active fine-scale buoyancy control. Taken together, these unprecedented results demonstrate that complex ecological behaviour can be achieved by "simple" organisms.