Foliar Application of Polymer-coated Manganese Dioxide Nanoparticles: Mechanisms of Uptake and Metabolic Responses in Manganese Deficient Barley

Pinna, A.; Gronbak, A. K.; Kristensen, E. V.; Thiebaut, N.; Szameitat, A.; Martin-Bertelsen, B.; Hassenkam, T.; Grivel, J.-C.; Mokso, R.; Husted, S.

doi:10.1101/2025.08.11.669670

bioRxiv · 10.1101/2025.08.11.669670

Foliar Application of Polymer-coated Manganese Dioxide Nanoparticles: Mechanisms of Uptake and Metabolic Responses in Manganese Deficient Barley

Pinna, A.·Gronbak, A. K.·Kristensen, E. V.·Thiebaut, N.·Szameitat, A.·Martin-Bertelsen, B.·Hassenkam, T.·Grivel, J.-C.·Mokso, R.·Husted, S.

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Abstract

The application of nanotechnology in plant science is unlocking innovative approaches to enhance nutrient use efficiency in crops, particularly through foliar fertilization. This study demonstrates that colloidally stable, pH-responsive polyacrylic acid (PAA)-coated manganese dioxide (MnO2) nanoparticles (nPAA-MnO2) can be designed to significantly restore key metabolic functionalities in manganese (Mn)-deficient barley (Hordeum vulgare) within a few days. Using a combination of advanced bioimaging techniques - including confocal laser scanning microscopy (CLSM), laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), and X-ray nano-computed tomography (nano-CT), we mapped the uptake and distribution pathways of nPAA-MnO2 compared to ionic Mn. While soluble Mn2+ ions primarily enter through hydrophilic cuticular pores, nPAA-MnO2 penetrates leaves via stomata, facilitated by the application of an organosilicone surfactant and glycerol to enhance wetting and hydraulic activation of stomatal pores. Within a few hours, nPAA-MnO2 accumulated in the sub-stomatal cavity and mesophyll apoplast, gradually releasing bioavailable Mn ions in the acidic apoplast environment. Moreover, labeling experiments with tracer ions revealed nPAA-MnO2 hot-spots around the vascular bundles and a limited but significant basipetal translocation of intact nanoparticles out of the foliar application zone, a pivotal step towards converting immobile nutrients such as Mn into mobile ones. Importantly, and unlike ionic Mn solutions, nPAA-MnO2 could be applied at high doses without causing leaf scorching and cytotoxicity, paving the way for more sustainable and efficient foliar fertilization practices. These novel aspects of nanoparticle uptake, translocation, and assimilation underscores the potential of nanotechnology to address nutrient mobility challenges in agriculture, representing an important contribution to the green transition of modern crop production.

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Pinna, A., Gronbak, A. K., Kristensen, E. V., Thiebaut, N., Szameitat, A., Martin-Bertelsen, B., Hassenkam, T., Grivel, J.-C., Mokso, R., Husted, S.. 2025-08-13. Foliar Application of Polymer-coated Manganese Dioxide Nanoparticles: Mechanisms of Uptake and Metabolic Responses in Manganese Deficient Barley. https://doi.org/10.1101/2025.08.11.669670

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