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bioRxiv · 10.1101/2025.08.06.668926

The Rapid Mechanically Activated (RMA) channel transduces increases in plasma membrane tension into transient calcium influx

Guerringue, Y.·Thomine, S.·Allain, J.-M.·Frachisse, J.-M.
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Abstract

O_LIPlants respond to mechanical stimuli by a rapid increase in cytosolic calcium. The intensity and kinetics of the calcium changes define calcium signatures important for biological responses . In this study, we determine the properties of a calcium permeable force-gated channel localized at the plasma membrane called Rapid Mechanically Activated (RMA). C_LIO_LIUsing patch-clamp and pressure-clamp, we characterized the kinetics of activation and inactivation of RMA channel upon stimulation by pulses of pressure applied onto the plasma membrane. Combining repetitive pressure pulse protocols at different frequencies with modeling, we investigated the channels capacity to transduce high frequency mechanical stimuli. C_LIO_LIRMA channel rapidly activates in response to membrane tension, then it inactivates during prolonged stimulation. Upon repeated stimulations, RMA current amplitude decreases irreversibly indicating that undergoes adaptation. The channel kinetics may be modeled with four chemical states and the model predicts that it behaves as a pass band filter in the 10 Hz - 1 kHz range. C_LIO_LIIn conclusion, due to its activation/inactivation characteristics, RMA channel is a candidate to mediate cytosolic calcium signaling in response to mechano-stimulation. Its adaptation and filtering properties suggest its involvement in the transduction of high frequency mechanical stimulation such as those produced by insects vibrations. C_LI

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Guerringue, Y., Thomine, S., Allain, J.-M., Frachisse, J.-M.. 2025-08-07. The Rapid Mechanically Activated (RMA) channel transduces increases in plasma membrane tension into transient calcium influx. https://doi.org/10.1101/2025.08.06.668926

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