Dilated cardiomyopathy remodels cardiac neuronal subtypes in a murine model

Sharma, S.

doi:10.1101/2025.08.05.668697

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bioRxiv · 10.1101/2025.08.05.668697

Dilated cardiomyopathy remodels cardiac neuronal subtypes in a murine model

Sharma, S.

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Abstract

The autonomic nervous system is comprised of two parallel chains sympathetic and parasympathetic nervous system that innervates myocardium heterogeneously. However, excessive sympathetic signalling is a hallmark of cardiac dysfunction including myocardial infarction and cardiomyopathies. In current research article, first, we carried out echocardiography on DCM-Tg9 mice to characterize cardiac output parameters and performed scRNAseq analysis in a dilated cardiomyopathy mouse model of heart-failure over wildtype control mice. We analysed differential gene expressions and performed pathway enrichment analysis. The important findings of our research indicate the increment within a specific neuronal subpopulation (NA1b) out of three adrenergic cardiac innervating neuronal subpopulation. We also observed significant increase in M-current potassium ion channel (Kcnq2 ion channel) and genes involved in pathways from oxidative phosphorylation and neurodegeneration pathways. These observations have clearly interpreted that significantly elevated expression of M-current ion channels might be involved with hyperactivated sympathetic signalling in heart-failure mice. HighlightsO_LIWe identified three adrenergic neuronal subtypes that innervate myocardium and one of these neuronal subtypes, NA1b, predominantly presents during the heart-failure. C_LIO_LIOur results indicated presence of m-current ion channels (Kcnq2) significantly elevated during heart failure in a murine dilated cardiomyopathy model. C_LIO_LIThe pathway enrichment analyses showed increased expression of genes that are involved in neurodegenerative and oxidative pathways. C_LI

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Sharma, S.. 2025-08-07. Dilated cardiomyopathy remodels cardiac neuronal subtypes in a murine model. https://doi.org/10.1101/2025.08.05.668697

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