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bioRxiv · 10.1101/2025.08.04.668534

DRUMBEAT: Temporally resolved interpretable machine learning model for characterizing state transitions in protein dynamics

Abstract

Conformational transitions are central to protein function, yet their mechanistic analysis remains challenging due to the multi-dimensionality and timescales underlying the molecular motions. While interpretable network models such as Bayesian networks have advanced the identification of key residue interactions in molecular dynamics (MD) data, they lack temporal resolution and cannot capture the sequence of events during transitions. Here, we introduce Dynamically Resolved Universal Model for BayEsiAn network Tracking or DRUMBEAT, a machine learning approach that combines a universal graph topology with sliding-window rescoring to generate interpretable, time-resolved maps of cooperative events in MD trajectories. Applying DRUMBEAT to the benchmark Fip35 WW domain folding trajectories from DE Shaw Research Group, we recover both major folding pathways and critical residues previously highlighted by experiment. Importantly, DRUMBEAT provides new insight in two ways: (1) uncover unknown protein features important for transition, and (2) dissect the order and timing of conformational changes, revealing the precise sequence of residue contact closures during individual folding events. Robustness analysis demonstrates that both the universal graph and time-resolved results are highly consistent across multiple sampling replicates. These findings establish DRUMBEAT as a scalable and interpretable machine learning framework for dissecting the dynamics of protein folding and other conformational transitions, offering a generalizable tool for the mechanistic study of biomolecular dynamics.

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BibTeXRIS

Manookian, B., Mukhaleva, E., Gogoshin, G., Bhattacharya, S., Vaidehi, N., RODIN, A. S., Branciamore, S.. 2025-08-04. DRUMBEAT: Temporally resolved interpretable machine learning model for characterizing state transitions in protein dynamics. https://doi.org/10.1101/2025.08.04.668534

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