In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells

Dendooven, T.; Ebrahimi, M.; dos Santos, A.; Dhondt, W.; Knowles, O.; Hale, T.; Burt, A.; Mehdipour, A. R.; Allegretti, M.

doi:10.1101/2025.08.04.668116

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bioRxiv · 10.1101/2025.08.04.668116

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells

Dendooven, T.·Ebrahimi, M.·dos Santos, A.·Dhondt, W.·Knowles, O.·Hale, T.·Burt, A.·Mehdipour, A. R.·Allegretti, M.

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Abstract

In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nature, their architecture in native membranes has been hitherto elusive. Here, we combine in-situ electron cryo-tomography, AlphaFold predictions and molecular dynamics simulations to determine the sub-nanometer-resolution architecture of the trimeric [~]130 kDa SUN5-Nesprin3 LINC complex in human spermatozoa. The structure reveals a hexagonal lattice that stitches the nuclear envelope, creating a rigid interface that links the sperm nucleus to the flagellum. Our structure unveils a unique membrane-anchoring mechanism: a SUN5 KASH-lid {beta}-hairpin and a Nesprin3 amphipathic helix insert into the outer nuclear membrane, leading to cooperative remodelling of the nuclear envelope shape by the extended LINC lattice, which results in flattening the caudal sperm nucleus. Our integrative data provides key insights into the architecture and role of a LINC complex in situ, revealing molecular details of a severe male infertility phenotype.

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Dendooven, T., Ebrahimi, M., dos Santos, A., Dhondt, W., Knowles, O., Hale, T., Burt, A., Mehdipour, A. R., Allegretti, M.. 2025-08-04. In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells. https://doi.org/10.1101/2025.08.04.668116

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