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bioRxiv · 10.1101/2025.07.31.667905

Benzo(a)pyrene degradation induces coordinated antioxidant and detoxification responses in the marine yeast Debaryomyces hansenii

Abstract

Polycyclic aromatic hydrocarbons (PAHs), such as benzo(a)pyrene (BaP), are persistent environmental pollutants recognized for their high toxicity and resistance to microbial degradation. In the search for efficient and promising organisms in mycoremediation, the marine yeast Debaryomyces hansenii has emerged as a compelling candidate, due to its ability to thrive under a variety of stressful conditions. This study presents the first integrated molecular and biochemical characterization of BaP degradation in a marine yeast, providing mechanistic insights into the detoxification and antioxidant responses involved. In this study, we explored D. hanseniis capacity to degrade BaP and characterized the associated molecular and biochemical responses induced by this compound. Our findings demonstrate that D. hansenii can tolerate BaP concentrations up to 100 ppm without compromising cell viability and is capable of degrading nearly 70% of the compound within six days. The degradation process appears to be enzymatically mediated, primarily involving cytochrome P450 (CYP), epoxide hydrolase (EH), and glutathione S-transferase (GST), enzymes typically associated with xenobiotic metabolism, and reactive oxygen species (ROS) generation. BaP exposure resulted in pronounced oxidative stress, evidenced by elevated ROS levels, lipid peroxidation, and protein carbonylation. However, D. hansenii activated a robust antioxidant defense, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and regulation of the glutathione redox system. Altogether, our data unveil a tightly coordinated cellular strategy that combines oxidation, conjugation, and detoxification pathways to counteract BaP-induced toxicity and sustain redox homeostasis. These insights position D. hansenii as a promising and metabolically adaptive organism for bioremediation of PAH-contaminated environments.

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BibTeXRIS

Padilla-Garfias, F., Araiza-Villanueva, M. G., Calahorra, M., Sanchez, N. S., Pena, A.. 2025-07-31. Benzo(a)pyrene degradation induces coordinated antioxidant and detoxification responses in the marine yeast Debaryomyces hansenii. https://doi.org/10.1101/2025.07.31.667905

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