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bioRxiv · 10.1101/2025.07.28.667293

Maternal Thyroid Supplementation Prevents Autistic-relevant Social Behavior and Hypothalamic Oxytocin Depletion Produced by Developmental Exposure to Environmental Toxicants

Kozlova, E. V.·Denys, M. E.·Bishay, A. E.·Luna, C. N.·De Angelis, M.·Campoy, L.·Habbal, A.·Lam, A. A.·Luvsanravdan, N.·Ghilenschi Colton, A.·Carter, D.·Mueller, T. D.·Schramm, K.-W.·Curras-Collazo, M.
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Abstract

Environmental toxicants that target the developing brain are suspected of contributing to autism spectrum disorder risk but causative evidence is lacking. We and others have shown that the indoor flame retardants, polybrominated diphenyl ethers (PBDEs), reduce prosocial behavior, however, few studies have assessed the central targets and underlying mechanisms. PBDEs are well established endocrine disruptors of the expanded thyroid system, which also regulates the prosocial neuropeptides oxytocin (OXT) and vasopressin (AVP) and their hypothalamic signaling. The potential role of PBDE-induced thyroid hormone (TH) deregulation in mediating disruption of central OXT and ASD-like social behavior deficits remains unmapped. To address this gap, we conducted a study in C57BL6/N mice that examined behavioral and neuromolecular reprogramming after developmental exposure to the commercial PBDE mixture, DE-71, and evaluated the therapeutic potential of TH supplementation. Dams were exposed daily during gestation and lactation to corn oil vehicle, low dose (0.1 mg/kg) and high dose (0.4 mg/kg) of DE-71 with or without concurrent L-thyroxine (+mT4). In offspring, dose-dependent ASD-relevant behavioral responses and central neuroendocrine OXT neuron depletion after developmental PBDE exposure was prevented with mT4. mRNA transcripts for the TH transporter Mct8, deiodinase (Dio3) and estrogen receptor beta (Esr2) expressed on OXT neurons in PVH were upregulated in low dose females. In contrast, Mct8 and Dio3 were downregulated in low dose males. These findings uncover sex-specific mechanisms of PBDE-induced reprogramming of TH-regulated pathways in hypothalamic neuroendocrine cells leading to depleted central OXT signaling and ultimately ASD-relevant phenotypes. Importantly, we provide novel evidence of the therapeutic potential of maternal thyroid supplementation against toxicant-induced neurodevelopmental disorders.

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Kozlova, E. V., Denys, M. E., Bishay, A. E., Luna, C. N., De Angelis, M., Campoy, L., Habbal, A., Lam, A. A., Luvsanravdan, N., Ghilenschi Colton, A., Carter, D., Mueller, T. D., Schramm, K.-W., Curras-Collazo, M.. 2025-07-31. Maternal Thyroid Supplementation Prevents Autistic-relevant Social Behavior and Hypothalamic Oxytocin Depletion Produced by Developmental Exposure to Environmental Toxicants. https://doi.org/10.1101/2025.07.28.667293

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