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bioRxiv · 10.1101/2025.07.24.666645

Voltage Imaging with Periodic Structured Illumination

Speed, F.·Teel, A.·Futia, G. L.·Restrepo, D.·Gibson, E. A.
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Abstract

We utilize periodic structured illumination with pseudo-HiLo (pHiLo) image reconstruction for in vivo voltage imaging. We demonstrate reduced signal from out-of-focus cells, that contaminates voltage activity for in-focus cells of interest, with pHiLo compared to traditional widefield recordings taken with uniform illumination and pseudo-widefield (pWF) reconstructions. We discuss tradeoffs between signal-to-background ratio, signal-to-noise ratio and temporal resolution for pHiLo in the context of high-speed voltage imaging in awake mice.

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Speed, F., Teel, A., Futia, G. L., Restrepo, D., Gibson, E. A.. 2025-07-30. Voltage Imaging with Periodic Structured Illumination. https://doi.org/10.1101/2025.07.24.666645

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