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bioRxiv · 10.1101/2025.07.24.666629

Huntingtin preserves mitochondrial genome integrity in neurons, which is impaired in Huntingtons disease

Abstract

Huntingtin (HTT) function is enigmatic, as the native protein plays critical roles in neuronal health, while mutant HTT (mHTT), carrying an expanded polyglutamine stretch, triggers neurotoxicity and contributes to the pathogenesis of Huntingtons disease (HD). We recently found that HTT is part of a nuclear transcription-coupled DNA repair (TCR) complex with DNA repair enzymes including polynucleotide-kinase-3-phosphatase (PNKP). This complex resolves DNA lesions during transcription to maintain genome integrity, while in HD, mHTT impairs the activity of this complex, resulting in accumulation of DNA lesions. Using molecular, cellular biology and computational methods, we find that HTT has a role in assembling a functional DNA repair complex in mitochondria. Together with mitochondrial RNA polymerase and transcription factors, HTT resolves mitochondrial DNA lesions to preserve mitochondrial genome integrity and function. Pathogenic mHTT impairs this activity, resulting in persistent DNA lesions and reduced mitochondrial function in HD. Importantly, restoring activity of this complex in a Drosophila HD model through ectopic HTT or PNKP expression significantly improves mitochondrial genome integrity and ameliorates motor deficits. HIGHLIGHTSO_LIHTT organizes a functional, multifactorial mitochondrial DNA repair complex C_LIO_LIMutant HTT impairs the mitochondrial DNA repair complex causing DNA damage accumulation C_LIO_LIHTT-associated repair complex resolves mitochondrial DNA lesions and DNA integrity C_LIO_LIRestoring repair activity in HD flies rescues mitochondrial DNA integrity and motor defects C_LI

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BibTeXRIS

Pradhan, S., Gaikwad, S., Tsai, C.-L., Smith, C., Zhang, N., Bush, K., Chakraborty, A., Yuan, S., Choudhary, S., Keene, C. D., Ellerby, L. M., Hazra, T. K., La Spada, A. R., Wairkar, Y. P., Ashizawa, T., Tainer, J. A., Pandita, T. K., Thompson, L. M., Sakkar, P. S.. 2025-07-24. Huntingtin preserves mitochondrial genome integrity in neurons, which is impaired in Huntingtons disease. https://doi.org/10.1101/2025.07.24.666629

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