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bioRxiv · 10.1101/2025.07.24.666371

Single cell decomposition of multicellular aging programs associated with impaired lung regeneration

Abstract

Aging impairs the regenerative capacity of mammalian organs and is a major risk factor for organ fibrosis. Mechanisms underlying persistent fibrosis after lung injury in old individuals remain unclear. We used longitudinal single-cell RNA-seq after lung injury and dissected aging effects computationally and experimentally at baseline and during repair. In old mice, sustained fibroblast activation in the resolution phase of fibrosis was associated with prolonged epithelial senescence and persistent epithelial-mesenchymal crosstalk. Single-cell interpretable tensor decomposition analysis revealed that aging most strongly affected T/B-lymphocytes and macrophages. Notably, we identified a Granzyme K-high CD8+ T cell state that was unique to aged mice, co-localized with epithelial progenitors, and its co-culture or Gzmk treatments in lung organoids impaired progenitor function by inducing stem cell senescence. In summary, our study highlights the effects of immune aging on epithelial progenitor function and provides a time-resolved high resolution map of lung regeneration in the context of aging.

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BibTeXRIS

Gote-Schniering, J., Melo-Narvaez, M. C., Boosarpu, G., Lauer, D., Prakki, S. R. S., Wang, H., Brunner, M., Khan, S., Ansari, M., Ammeter, M., Jain, E., Puda, N., Wiedemann, K., Steinchen, C., Ashgapour, S., Eceiza, A., Dudek, M., Mayr, C. H., Chen, Y., Angelidis, I., Yildirim, O., Knolle, P., Burgstaller, G., Koenigshoff, M., Kadri, S., Mueck-Haeusl, M., Theis, F. J., Schiller, H. B., Lehmann, M.. 2025-07-28. Single cell decomposition of multicellular aging programs associated with impaired lung regeneration. https://doi.org/10.1101/2025.07.24.666371

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