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bioRxiv · 10.1101/2025.07.21.666022

Non-invasive brain stimulation protects cognitive impairment in i.c.v.STZ injected rats: role of adult neurogenesis

Kumar, A.·Roy, A.·Karaddi, V.·Jain, S.·Katyal, J.·Gupta, Y. K.
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Abstract

In Alzheimers like dementia, neurodegeneration and synaptic dysfunction is known to the critical player in explaining the cognitive impairment. Adult neurogenesis, normally a chronic and quite process is explained to have potential in the field of Alzheimers therapy. Previous research on the non-invasive brain stimulation showed that controlling pattern of stimulation externally we can regulate/ entrain neuronal activity, possibly altering the structural changes in the circuit level. However, literature investigating if non-invasive brain stimulation could hold any potential to initiate process of adult neurogenesis are scarce. In the present study, with the use of behavioural, microscopic and biochemical tools we found that extremely low frequency magnetic field at an intensity of 17.96{micro}T with sinusoidal wave of 50Hz for 2hr daily for a period of two week in streptozotocin induced animal model of sporadic Alzheimers disease can cause improvement in spatial and reference memory, influencing their swimming strategy in water maze. Which is caused by stimulation in immature neural pluripotent stem cells, with additional redox balance and mitigation of glial aggravation in brain areas like olfactory bulb, prefrontal cortex and hippocampus. These changes were accompanied by neuroprotection as observed in the granular layer of dentate gyrus. Taken together, present study explains plausible mechanism of action on protection of neurodegeneration in Alzheimers disease through non-invasive brain stimulation. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=91 SRC="FIGDIR/small/666022v1_ufig1.gif" ALT="Figure 1"> View larger version (29K): org.highwire.dtl.DTLVardef@bc7830org.highwire.dtl.DTLVardef@176cc01org.highwire.dtl.DTLVardef@4b869dorg.highwire.dtl.DTLVardef@72e703_HPS_FORMAT_FIGEXP M_FIG Graphical illustration representing external magnetic field causing cognitive improvement eliciting adult neurogenesis in animal model of Alzheimers disease C_FIG

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Kumar, A., Roy, A., Karaddi, V., Jain, S., Katyal, J., Gupta, Y. K.. 2025-07-25. Non-invasive brain stimulation protects cognitive impairment in i.c.v.STZ injected rats: role of adult neurogenesis. https://doi.org/10.1101/2025.07.21.666022

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