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bioRxiv · 10.1101/2025.07.21.665917

Protein Malnutrition Facilitates Intestinal Colonization with Highly Resistant Klebsiella pneumoniae

Abstract

Pediatric infections with Highly Resistant Enterobacterales (HRE), including Klebsiella pneumoniae resistant to 3rd-generation cephalosporins and/or carbapenems, disproportionately affect low- and middle-income countries where malnutrition is prevalent. The underlying mechanisms linking malnutrition to HRE colonization in children have not been established. In this study we developed a mouse model of pediatric malnutrition and intestinal colonization with clinical isolates of carbapenem-resistant K. pneumoniae (CR-Kp). Juvenile mice fed a protein-deficient diet (PD) were more susceptible to intestinal colonization after inoculation with human-derived strains of CR-Kp, demonstrating a 3-4 log higher colonization burden in comparison to mice fed a control diet (CD). Colonization in PD-fed mice persisted for up to 6 weeks and CR-Kp were transmitted between PD-fed but not CD-fed cage mates. Antibiotic treatment resulted in similar CR-Kp colonization burdens regardless of diet, suggesting that nutrition-dependent colonization resistance is reliant on an intact microbiota. Secondary bile acids, a product of resident intestinal microbiota, were reduced in PD-fed and antibiotic treated mice and demonstrated an inverse correlation with CR-Kp burden. Secondary bile acids directly inhibited CR-Kp growth in vitro, suggesting that a loss of these inhibitory metabolites may mediate malnutrition-induced susceptibility to HRE colonization.

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BibTeXRIS

Holowka, T., Santiago, V. F., Xiao, J., Liccione, M. F., Bimagambetov, A., Walsh, K., Arnold, J. W., Marimuthu, K., Ng, O. T., Swann, J., van Duin, D., Bartelt, L. A.. 2025-07-21. Protein Malnutrition Facilitates Intestinal Colonization with Highly Resistant Klebsiella pneumoniae. https://doi.org/10.1101/2025.07.21.665917

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