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bioRxiv · 10.1101/2025.07.15.664741

Inconsistent short-term effects of enhanced structural complexity on soil microbial properties across German forests

Schwarz, R.·Eisenhauer, N.·Ammer, C.·Bradler, P. M.·Decker, O.·Delory, B. M.·Dietrich, P.·Fichtner, A.·Huang, Y.·Lettenmaier, L.·Junginger, M.·Mitesser, O.·Mueller, J.·von Oheimb, G.·Pierick, K.·Scherer-Lorenzen, M.·Cesarz, S.
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Abstract

Structural and biotic homogenization can result from forestry practices that lack promotion of canopy gaps and deadwood. This can lead to biodiversity loss and impaired ecosystem functions. Enhancing structural complexity (ESC) has been proposed to counteract these effects, but its impact on soil properties remains insufficiently understood. Overall, we hypothesize that ESC enhances soil abiotic properties, their spatial variability, and microbial functioning, with effects modulated by environmental context and increasing over time. Data were collected from 148 patches (50 x 50 m) in eight beech forests across Germany. In half of the patches, structural complexity was enhanced by felling 30% of the basal area of living trees through two spatial patterns--aggregated (one large gap) and distributed (small gaps)--combined with leaving or removing deadwood (stumps, logs, and snags). The other half served as controls, representing typically managed, homogeneous production forests. Soil C:N, C%, and N% increased near deadwood. Soil microbial biomass and activity were significantly affected in three of eight forest sites, with effects ranging from -30% to +62%. Higher soil water content was associated with increased microbial biomass, and greater understorey biomass correlated with a lower microbial respiratory quotient. No temporal trends were observed over five years. Although soil properties showed resistance to structural interventions, site-specific effects underline the importance of soil moisture and the understorey vegetation for microbial functioning. Further research building on our results is needed to develop practical forest management strategies to clarify how structural complexity may support soil functioning and ecosystem resilience. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=127 SRC="FIGDIR/small/664741v1_ufig1.gif" ALT="Figure 1"> View larger version (53K): org.highwire.dtl.DTLVardef@10790f2org.highwire.dtl.DTLVardef@1bd2f95org.highwire.dtl.DTLVardef@17eed6dorg.highwire.dtl.DTLVardef@15a049_HPS_FORMAT_FIGEXP M_FIG C_FIG Highlights- Deadwood addition increases soil C%, N%, and the soil C:N ratio - Enhanced structural complexity alters soil microbial properties in site-specific ways - Soil water content changes are linked to shifts in microbial biomass - Understorey biomass changes are linked to shifts in the respiratory quotient

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Schwarz, R., Eisenhauer, N., Ammer, C., Bradler, P. M., Decker, O., Delory, B. M., Dietrich, P., Fichtner, A., Huang, Y., Lettenmaier, L., Junginger, M., Mitesser, O., Mueller, J., von Oheimb, G., Pierick, K., Scherer-Lorenzen, M., Cesarz, S.. 2025-07-18. Inconsistent short-term effects of enhanced structural complexity on soil microbial properties across German forests. https://doi.org/10.1101/2025.07.15.664741

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