Endogenous glucocorticoids moderate the gastric inflammatory response to Helicobacter infection and protect from autoimmunity.

Druffner, S. R.; Duncan, B. C.; Morris, M. T.; Pascoe, J. L.; Abner, T. M.; Hussain, S.; Piazuelo, M. B.; Peek, R. M.; Zhang, M.; DiPaolo, R. J.; Busada, J. T.

doi:10.1101/2025.07.03.662976

bioRxiv · 10.1101/2025.07.03.662976

Endogenous glucocorticoids moderate the gastric inflammatory response to Helicobacter infection and protect from autoimmunity.

Druffner, S. R.·Duncan, B. C.·Morris, M. T.·Pascoe, J. L.·Abner, T. M.·Hussain, S.·Piazuelo, M. B.·Peek, R. M.·Zhang, M.·DiPaolo, R. J.·Busada, J. T.

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Abstract

Background and AimsImmune responses to infection must balance pathogen clearance with minimizing tissue damage and autoimmunity. Chronic gastric inflammation caused by H. pylori damages the gastric mucosa and promotes carcinogenesis. Glucocorticoids are immunoregulatory hormones that limit immune activation in the stomach. This study aimed to determine how endogenous glucocorticoids regulate the gastric immune response to Helicobacter infection and their impact on preneoplastic lesion development. MethodsWe examined the role of endogenous glucocorticoids in shaping the gastric immune response to Helicobacter felis colonization. Gastric immune cell infiltration, atrophy, metaplasia, and preneoplastic lesion development were evaluated in adrenal-intact control mice and adrenalectomized (ADX) mice. Auto-reactive IgG antibodies were assessed using a mouse self-antigen array and by measuring their binding to healthy gastric tissue. ResultsLoss of endogenous glucocorticoids led to significantly increased H. felis-induced gastric T cell infiltration and proinflammatory cytokine expression compared to intact-infected controls. While all intact mice maintained chronic infection for up to 12 months, nearly all ADX mice eradicated H. felis within 2-3 weeks. Despite bacterial clearance, ADX mice continued to exhibit chronic gastric inflammation and developed dysplasia. Autoantibody profiling showed that both intact and ADX groups generated self-reactive IgG during active infection. However, only ADX mice sustained autoantibody production following bacterial eradication. ConclusionsEndogenous glucocorticoids attenuate gastric inflammation during Helicobacter infection, supporting bacterial persistence while maintaining immune tolerance. These findings suggest that heightened immune responses to H. pylori may trigger autoimmune gastritis (AIG) development, which can persist after H. pylori clearance and continue to drive gastric cancer risk.

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Druffner, S. R., Duncan, B. C., Morris, M. T., Pascoe, J. L., Abner, T. M., Hussain, S., Piazuelo, M. B., Peek, R. M., Zhang, M., DiPaolo, R. J., Busada, J. T.. 2025-07-06. Endogenous glucocorticoids moderate the gastric inflammatory response to Helicobacter infection and protect from autoimmunity.. https://doi.org/10.1101/2025.07.03.662976

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