The systemic JIA synovial fluid environment supports development and prevalence of specific inflammatory T helper cell phenotypes

Schleifenbaum, S.; Swoboda, A.; Dirks, J.; Bracaglia, C.; Caiello, I.; Hinze, T.; Hardt, S.; Prencipe, G.; Tarantino, G.; Pardeo, M.; Fuehner, S.; Park, C.; Hinze, C.; Wittkowski, H.; Windschall, D.; Foell, D.; Morbach, H.; Kessel, C.

doi:10.1101/2025.07.01.661738

bioRxiv · 10.1101/2025.07.01.661738

The systemic JIA synovial fluid environment supports development and prevalence of specific inflammatory T helper cell phenotypes

Schleifenbaum, S.·Swoboda, A.·Dirks, J.·Bracaglia, C.·Caiello, I.·Hinze, T.·Hardt, S.·Prencipe, G.·Tarantino, G.·Pardeo, M.·Fuehner, S.·Park, C.·Hinze, C.·Wittkowski, H.·Windschall, D.·Foell, D.·Morbach, H.·Kessel, C.

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Abstract

ObjectiveThe potential involvement of adaptive immunity in systemic juvenile idiopathic arthritis (sJIA) pathophysiology remains an intriguing question. Here, we investigated whether and how the inflammatory environment in sJIA versus JIA synovial fluid (SF) may differentially impact T helper (Th) cell polarization and activation. MethodsSF samples from sJIA and JIA patients (both n=7) were tested in various cell culture setups, with or without recombinant cytokines or cytokine-blocking drugs, to assess their effects on healthy donor Th cell activation. We analyzed cellular surface marker, transcription factor, and effector molecule expression using flow cytometry, Luminex, ELISA, and qRT-PCR. ResultsBoth sJIA and JIA SF revealed highly pro-inflammatory profiles. Compared to JIA, sJIA SF demonstrated markedly elevated IL-1{beta}, IL-18, GM-CSF, S100A9, and MPO levels, while JIA SF showed trends toward higher soluble FasL and IL-17A concentrations. Notably, sJIA SF significantly increased CD4 T cell ICOS expression and expanded CXCR3posCCR6pos Th cells, whereas JIA SF favored expansion of CXCR3negCCR6pos Th cells and CCR6pos Th cell expansion was sensitive to IL-1 blockade. Systemic JIA SF selectively sustained a IFN{gamma}/IL-21 expressing T peripheral helper (Tph) phenotype, particularly associated with IL-1{beta}, IL-18, and GM-CSF SF levels. Spiking JIA SF with a cocktail of these cytokines recapitulated some T cellular phenotypic features observed in sJIA SF cultures. ConclusionJIA and sJIA SF drive distinct Th cell polarization, including differential and sustained Tf/ph cell activation. These findings complement our earlier observations in sJIA peripheral blood and demonstrate the impact of the SF inflammatory matrix on immune cell activation. What is already known on this topicO_LISystemic juvenile idiopathic arthritis (sJIA, Stills Disease) is initially hallmarked by innate immunity driving systemic inflammation C_LIO_LIWith further disease course sJIA can progress to chronic destructive arthritis with several studies suggesting an involvement of adaptive immunity in this process. C_LIO_LIIn a previous study we linked T follicular/peripheral helper (Tfh/Tph) cells in sJIA patients blood to arthritis and self-reactive antibody signatures in patients with longer disease duration C_LI What this study addsO_LIOur study demonstrates that sJIA versus JIA synovial fluid (SF) can drive differential T helper cell (Th cell) polarization and activation. C_LIO_LIOur data imply that sJIA SF promotes the self-sustenance of Th cells with a Tph phenotype characterized by IFN{gamma}, IL-21 and c-MAF expression. C_LIO_LIIL-1{beta}, IL-18, and GM-CSF levels in sJIA SF can be associated with Tph perseverance, and recapitulate sJIA Tph features when spiked into JIA SF. C_LI How this study might affect research, practice or policyO_LIThe present data complement our earlier observations from sJIA peripheral blood and strengthen the biphasic model hypothesis regarding sJIA progression C_LIO_LIWhile both JIA and sJIA patients can develop clinically similar arthritis, our data demonstrate how the respective local inflammatory environments can differentially impact and shape T cell immunity. C_LIO_LIOur data suggest a combined and early targeting of both IL-1{beta} and IL-18 in sJIA may be effective in preventing the generation of inflammatory T cell subsets with the potential to drive chronic arthritis through a joint-localized, adaptive immune response. C_LI

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Schleifenbaum, S., Swoboda, A., Dirks, J., Bracaglia, C., Caiello, I., Hinze, T., Hardt, S., Prencipe, G., Tarantino, G., Pardeo, M., Fuehner, S., Park, C., Hinze, C., Wittkowski, H., Windschall, D., Foell, D., Morbach, H., Kessel, C.. 2025-07-04. The systemic JIA synovial fluid environment supports development and prevalence of specific inflammatory T helper cell phenotypes. https://doi.org/10.1101/2025.07.01.661738

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