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bioRxiv · 10.1101/2025.06.27.661933

M18BP1 valency and a distributed interaction footprint determine epigenetic centromere specification in humans

Walstein, K.·Hill, L.·Vogt, D.·Vetter, I. R.·Pan, D.·Musacchio, A.
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Abstract

Deposition of CENP-A, the histone H3 variant considered an epigenetic landmark of centromeres, reflects cell-cycle-regulated assembly of M18BP1, HJURP, and PLK1 on a divalent MIS18/{beta} scaffold. The localization determinants of this machinery remain poorly characterized. Here we report that artificial M18BP1 dimerization bypasses MIS18/{beta}, allowing the identification of at least four determinants of M18BP1 centromere localization. These include the SANTA domain, of which we report the first structure, as well as linear motifs in disordered neighboring regions, of which we characterize the interaction footprint on the CENP-A associated 16-subunit constitutive centromere-associated network (CCAN). Our observations imply that M18BP1, after dimerization, is necessary and sufficient for centromere localization. Its cell-cycle-dependent dimerization on MIS18/{beta} promotes initial recognition of a multivalent centromeric assembly of old CENP-A and associated proteins, followed by cooption of PLK1 and HJURP and new CENP-A deposition. Our results shed new light on the determinants of centromere epigenetic inheritance in humans.

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Walstein, K., Hill, L., Vogt, D., Vetter, I. R., Pan, D., Musacchio, A.. 2025-06-30. M18BP1 valency and a distributed interaction footprint determine epigenetic centromere specification in humans. https://doi.org/10.1101/2025.06.27.661933

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