bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2025.06.27.661925

Alpha-2-Macroglobulin mitigates mitochondrial dysfunction and neuronal apoptotic responses in pesticide-induced neurotoxicity

Dixit, S.
View original source ↗Export metadata ↓

Abstract

Persistent and repetitive application of pesticides has been linked to adverse effects on human metabolism and the onset of various disorders. Commonly used pesticides, such as carbamates (e.g., Aldicarb, ALD) and organophosphates (e.g., Chlorpyrifos, CPF), are widely applied in potato cultivation and household pest control. Chronic exposure to these substances has been implicated in the early onset of neurodegenerative disorders. Alpha-2-macroglobulin (A2M), a protein known for its regulatory role in oxidative stress, participates in multiple biological processes. Despite its significance, the role of A2M in mitigating mitochondrial-induced neuronal apoptosis triggered by pesticide interference remains poorly understood. This study explores the involvement of A2M in SH-SY5Y neuroblastoma cells exposed to pesticides, focusing on its impact on mitochondrial enzyme expression, inflammatory cytokines, neuronal apoptotic and anti-apoptotic proteins, and the activation of the Nrf2 signaling pathway. Comparative analyses of control and pesticide-exposed SH-SY5Y cells revealed that A2M positively modulates neuronal stress responses. Western blot profiling demonstrated that A2M upregulates anti-apoptotic proteins such as Bcl-2 and Nrf2, while downregulating pro-apoptotic markers, including Bax, Caspase-3, and Caspase-9. Biochemical assays showed that A2M enhances mitochondrial enzyme activity, particularly complexes I and III, while mitigating the reactive oxygen species (ROS) generated by ALD and CPF exposure. Furthermore, A2M was found to reduce DNA damage caused by pro-inflammatory cytokines, which are exacerbated by mitochondrial oxidative stress. These findings highlight the pivotal role of A2M in attenuating pesticide-induced neuronal toxicity through the regulation of mitochondrial function and inhibition of neuronal apoptosis. Research HighlightsO_LIThis study is the first to report the interaction between human alpha-2-macroglobulin and neuronal apoptotic markers in pesticides mediated neurotoxicity. C_LIO_LIThe study found that treatment of alpha-2-macroglobulin promotes the proliferation of Nrf2 pathway during oxidative stress in neuronal cells. C_LIO_LIThis research reveals that alpha-2-macroglobulin modulates oxidative stress in mitochondrial enzyme complexes and during mitochondrial dysfunction. C_LIO_LIThe study confirms that treatment of alpha-2-macroglobulin significantly alleviates pro inflammatory cytokines during pesticides mediated neurotoxicity. C_LIO_LIThis research provides new theoretical basis and molecular targets for the treatment of neurodegenerative diseases during early preclinical stages, offering broad application prospects. C_LI GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=161 SRC="FIGDIR/small/661925v1_ufig1.gif" ALT="Figure 1"> View larger version (45K): org.highwire.dtl.DTLVardef@1acb2baorg.highwire.dtl.DTLVardef@109ead0org.highwire.dtl.DTLVardef@171f0borg.highwire.dtl.DTLVardef@1f745b1_HPS_FORMAT_FIGEXP M_FIG C_FIG

Source connections

Explore related subjects

biochemistryPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Dixit, S.. 2025-06-27. Alpha-2-Macroglobulin mitigates mitochondrial dysfunction and neuronal apoptotic responses in pesticide-induced neurotoxicity. https://doi.org/10.1101/2025.06.27.661925

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Hierarchical cysteine oxidation controls reversible amyloid formation in an ankyrin repeat protein

The formation of amyloids, including functional amyloids, is observed for an increasing number of proteins but the molecular mechanisms that control this structural transition remain poorly understood. Here we report that the kinase inhibitor protein P18 (drP18) from Danio rerio (zebrafish), which contains two cysteine residues, undergoes a complex and hierarchical redox switch that strictly governs reversible amyloid formation. We identify cysteine 50 (C50) acting as a regulatory residue. Upon oxidation, C50 forms an intramolecular disulfide bond with the executioner cysteine 128 (C128), thereby blocking it. C50 can become S-glutathionylated, and upon oxidation, C128 then forms intermolecular disulfides that lead to rapid transition into amyloid fibrils. S-glutathionylation of C50 therefore enables amyloid formation of drP18 and the outcome is oxidant-dependent with diamide, hydrogen peroxide, peroxymonocarbonate and hypothiocyanous acid each leading to amyloid assembly with distinct kinetics and morphologies. These amyloids are fully reversible, where disulfide reduction is leading to disassembly. Whereas monomeric drP18 inhibits CDK4-mediated retinoblastoma phosphorylation, the amyloid conformation abolishes this inhibition, and reduction restores both structure and function. Expression of drP18 in zebrafish embryos yields Congo red-positive, oxidation-dependent aggregates in vivo. Together, our findings show that a regulatory cysteine controls an executioner cysteine to induce reversible, functional amyloid formation, revealing that proteins can encode sophisticated mechanisms to control amyloid assembly.

biochemistry↗
bioRxiv2026

Snapshots from the Catalytic Landscape of Chalcone Isomerase

Chalcone isomerase (CHI) catalyzes the cyclization of 3-ring scaffolds of flavonoids, a class of plant-based natural products important for nutrition and disease prevention. A persistent question has been whether the enzyme uses dynamics to facilitate conformational rearrangements of substrates within the active site. To help resolve this question, CHI was crystallized with phloretin, a flexible substrate analogue that cannot undergo cyclization. The crystal structure possesses eight protein molecules per asymmetric unit, revealing different active site conformations that accommodate different bound conformers of phloretin. Together, the structural snapshots depict a series of coordinated, dynamic chemical interactions that lower barriers to substrate rearrangements approaching bond formation. Differential scanning fluorimetry combined with mutational analysis and enzyme kinetics further confirm that phloretin binds to the enzyme active site and that it acts as a competitive inhibitor of CHI. Together these findings answer outstanding questions about the flexibility and dynamics of CHI catalysis, information that may be useful for future biosynthetic design and enzyme engineering goals. Overall, this work supports a catalytic model in which the CHI enzyme operates as a dynamic ensemble of structures necessary to facilitate catalytic substrate rearrangements.

biochemistry↗
bioRxiv2026

Structures of pUG-fold RNA bound to DNMT1 reveal a mechanism for RNA-mediated epigenetic regulation

Many chromatin-associated proteins have been found to bind RNA as a means of epigenetic regulation. Specifically, DNA methyltransferase 1 (DNMT1), which maintains cytosine methylation at CpG dinucleotides, is inhibited by RNA at transcribed DNA loci in cells. However, the mechanisms by which RNA binds DNMT1 and inhibits its activity remain unknown. Here, we determine a series of cryogenic electron microscopy (cryo-EM) structures of human DNMT1 bound to pUG-fold RNA, a non-canonical G-quadruplex previously observed to inhibit activity, revealing two distinct RNA-binding modes. The pUG-fold RNA binds the surface of DNMT1 in its autoinhibited conformation across a positively charged surface between the methyltransferase domain and the CXXC domain, and it binds directly in the active site of an open DNMT1 conformation. RNA binding is sterically incompatible with substrate DNA engagement in both states. Our 2.5 [A] structure captures the intricate network of hydrogen bonds and electrostatic interactions between amino acids in the methyltransferase domain and the tetrad layers of pUG-fold RNA. Metadynamics molecular dynamics simulations provide an orthogonal view of the conformational landscape of DNMT1, revealing the two distinct RNA-binding modes. Furthermore, our analysis of published DNMT1 RIP-seq and eCLIP-seq data confirms that DNMT1-interacting RNAs in cells exhibit a strong propensity to form non-canonical G-quadruplex RNA structures. Collectively, our study provides the first structural basis for pUG-fold RNA recognition by a protein and illustrates how cryo-EM and AI-based methods for protein and RNA structure prediction synergize to inform the mechanism of RNA-mediated regulation of DNMT1.

biochemistry↗