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bioRxiv · 10.1101/2025.06.27.661900

N-Cadherin/α-Catenin Drive Adhesion and Actin Regulation to Orchestrate Tunneling Nanotube Formation

Zurzolo, C.·Belian, S.·Pepe, A.·Notario Manzano, R.·Sartori-Rupp, A.·Brou, C.
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Abstract

Cell-to-cell communication is essential for maintaining homeostasis in multicellular organisms. Tunneling nanotubes (TNTs)--actin-based membranous connections--mediate the exchange of diverse cargoes between distant cells. Unlike other cellular protrusions, TNTs exhibit unique ultrastructural features and are enriched in the adhesion molecule N-Cadherin. Here, we dissect the role of N-Cadherin in the formation and function of TNTs in SH-SY5Y human neuronal-like cells. We show that N-Cadherin, via its effectors -Catenin and p120-Catenin, is a central regulator of TNT architecture and their cargo transfer capability. Regulators of cortical tension p120-Catenin, ROCK, and non-muscle myosin II also emerge as critical for TNT functionality, highlighting a mechanosensitive component to TNT regulation. Moreover, we reveal that NMIIA can be processive inside TNTs and transfer through them using actins retrograde flow. Finally, we identify the Cdc42-IRSp53-N-WASP pathway as a downstream effector axis enhancing intercellular transfer downstream of N-Cadherin. Together, our findings uncover a structural and functional link between N-Cadherin signaling and TNT-mediated intercellular communication.

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Zurzolo, C., Belian, S., Pepe, A., Notario Manzano, R., Sartori-Rupp, A., Brou, C.. 2025-06-27. N-Cadherin/α-Catenin Drive Adhesion and Actin Regulation to Orchestrate Tunneling Nanotube Formation. https://doi.org/10.1101/2025.06.27.661900

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