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bioRxiv · 10.1101/2025.06.26.661592

Long-Term Effects of Adolescent 5F-MDMB-PICA Intravenous self-administration: Neurobehavioral Consequences and medial Prefrontal Cortex Dysfunction in Adult Mice

Caria, F.·Libster, A.·Desfor, S.·Telese, F.·Di Chiara, G.·De Luca, M. A.
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Abstract

BackgroundSynthetic Cannabinoids Receptor Agonists (SCRAs) are the largest group of new psychoactive substances monitored worldwide. 5F-MDMB-PICA is a recent SCRA classified as a potent full agonist at CB1/CB2 receptors able to activate the mesolimbic dopamine (DA) transmission in adolescent but not in adult mice. Here, we have studied its reinforcing effects in adolescent mice and characterized the neurochemical and behavioral effects induced in the same animals in adulthood. MethodsWe utilized an intravenous self-administration (IVSA) protocol in adolescent (PND 40-56) CD-1 male mice. In adulthood (PND 66-78), we conducted several behavioral and neurobiological assessments including: Sucrose Preference Test (SPT); Resident Intruder Test (RIT); Olfactory Reactivity Test (ORT); brain microdialysis to quantify DA levels in the medial Prefrontal Cortex (mPFC); and fiber photometry analysis using the GCaMP calcium sensor to monitor excitatory neural dynamics in the mPFC after exposure to an aversive odorant. ResultsWe found that 5F-MDMB-PICA, administered through IVSA in adolescent mice, produced an inverted U-shaped dose-response curve. The dose of 2.5 g/kg/25ul elicited behavior consistent with drug seeking. Adult mice exposed to 5F-MDMB-PICA during adolescence exhibited significant behavioral and neurochemical changes in adulthood compared to control mice. These behaviors included increased aggression, reduced social interaction, an anhedonic state, and an abolishment of mPFC DA response to an aversive odorant, as measured by in vivo brain microdialysis. Moreover, fiber photometry analysis of excitatory neuronal activity in the mPFC showed diminished calcium activity in response to the same aversive odorant in 5F-MDMB-PICA-exposed mice compared to controls. ConclusionsNotably, this study is the first to demonstrate that adolescent mice can acquire and sustain IVSA of 5F-MDMB-PICA. Furthermore, it highlights the long-term behavioral and neurochemical changes associated with adolescent exposure to 5F-MDMB-PICA, underscoring the potential detrimental effects of its use during this critical developmental period.

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Caria, F., Libster, A., Desfor, S., Telese, F., Di Chiara, G., De Luca, M. A.. 2025-06-29. Long-Term Effects of Adolescent 5F-MDMB-PICA Intravenous self-administration: Neurobehavioral Consequences and medial Prefrontal Cortex Dysfunction in Adult Mice. https://doi.org/10.1101/2025.06.26.661592

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