MAPseq2: a sensitive and cost-effective barcoded connectomics method

Kim, H.; Qi, H.; Washington, C.; Liang, X.; Kebschull, J. M.

doi:10.1101/2025.06.23.661165

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bioRxiv · 10.1101/2025.06.23.661165

MAPseq2: a sensitive and cost-effective barcoded connectomics method

Kim, H.·Qi, H.·Washington, C.·Liang, X.·Kebschull, J. M.

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Abstract

The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a format solvable by DNA sequencing. Here we present MAPseq2, a user-friendly protocol with up to [~]10-fold increased sensitivity compared to the current state of the art and [~]10-fold decreased cost. As MAPseq workflows are used across a range of barcoded connectomics methods, including BARseq, BRICseq, and ConnectID, all improvements in MAPseq2 directly transfer to these technologies.

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Kim, H., Qi, H., Washington, C., Liang, X., Kebschull, J. M.. 2025-06-24. MAPseq2: a sensitive and cost-effective barcoded connectomics method. https://doi.org/10.1101/2025.06.23.661165

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