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bioRxiv · 10.1101/2025.06.16.659826

Evaluating the Safety of Intravenous Injection of Nitric Oxide, Magnesium, and Hydrogen Nano Bubble Solution in Sprague Dawley Rats

Novrial, D.·Inayati, N. S.·Gumilas, N. S. A.·Kurniawan, D. W.·Sumitro, S. B.
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Abstract

Nanobubble technology exhibits outstanding stability and permeability and has been extensively developed in pharmaceuticals, dentistry, and medicine. The Hydrogen Nano Bubble (HNB) solution containing NO and magnesium is projected to have an even greater therapeutic effect than has ever been investigated. An experimental study using Sprague-Dawley rats was conducted to assess the intravenous injections safety. Each rat received a single injection into the tail vein at a dose of 2 mL. On the other hand, aquades were injected into the rats in the control group. Observations were made every 30 minutes for the first four hours, then every four hours for the first twenty-four hours, and once daily for the next fourteen days. The LD50, urea-creatinine level, SGOT/SGPT level, and organ histology, including liver, kidney, heart, lung, and spleen, were evaluated. The solution appears safe for intravenous administration, as there were no adverse side effects during the trial, and all values were still within the normal range. Additional investigation is required to assess the long-term harmful impact.

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Novrial, D., Inayati, N. S., Gumilas, N. S. A., Kurniawan, D. W., Sumitro, S. B.. 2025-06-20. Evaluating the Safety of Intravenous Injection of Nitric Oxide, Magnesium, and Hydrogen Nano Bubble Solution in Sprague Dawley Rats. https://doi.org/10.1101/2025.06.16.659826

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