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bioRxiv · 10.1101/2025.06.15.659808

Human parainfluenza virus infection remodels the host cell glycome

Das, P. K.·Dirr, L.·Bailly, B.·Guillon, P.·Everest-Dass, A.·von Itzstein, M.
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Abstract

Human parainfluenza virus type 3 (HPIV-3) remains a major cause of respiratory illness particularly among young children, the elderly and immunocompromised individuals. Despite significant efforts in therapeutic discovery research, there is neither an effective antiviral nor a vaccine available against HPIV-3. Host cell glycosylation is known to play a pivotal role in virus entry and replication. While some host glycan-based cellular receptors for HPIV-3 have been identified, the dynamics of the host glycome upon HPIV-3 infection has never been studied. Herein, we report the first mass spectrometry-based study that provides direct insight into the remodelling of the human lung adenocarcinoma cell (A549) glycome upon HPIV-3 infection. In this study we observed that HPIV-3 infection led to significant host-cell glycome changes in both oligomannose and sialylated complex-type N-glycans. Moreover, notable changes were also observed in both core 1 and core 2 type O-glycans, along with distinct glycosphingolipid remodelling in infected cells compared to their mock-infected counterparts. Our study presents the first evidence that hPIV-3 infection alters host-cell glycome, offering new insights into the viruss impact on host cellular processes.

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Das, P. K., Dirr, L., Bailly, B., Guillon, P., Everest-Dass, A., von Itzstein, M.. 2025-06-17. Human parainfluenza virus infection remodels the host cell glycome. https://doi.org/10.1101/2025.06.15.659808

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