Multiparent Recombinant Inbred lines crossed to a tester provide novel insights into sources of cis and trans regulation of gene expression

Abstract

We propose crossing multi-parent recombinant-inbred-lines (RILs) to a common tester and measuring allele specific gene expression in the offspring. Testing whether allelic imbalance between two RIL x Tester crosses is equal, is a test of cis or trans depending on the RIL alleles compared. The study design also enables to separate two sources of trans variation, genetic and environmental, detected via interactions with cis effects. Examining these components of regulatory variation, we demonstrate this approach in a long-read RNA-seq experiment in female abdominal tissue at two time points in Drosophila melanogaster. Among the 40% of all loci that show evidence of genetic variation in cis, trans effects due to the environment are detectable in 31% of loci and trans effects due to genetic background are detectable in 19% of loci with little overlap in sources of trans variation. The loci identified in this study are associated with loci previously reported to exhibit genetic variation in gene expression in a range of tissues and large population samples, suggesting that there is consistent variation for genetic regulation of gene expression. We show that eleven loci in a QTL for thermotolerance, previously shown to differ in expression based on temperature, have evidence for regulation of gene expression regardless of the environment, including Cpr67B, a cuticular protein suggesting a potential functional role for standing variation in gene expression. This study provides a blueprint for efficiently identifying regulatory variation in gene expression, as the tester design maximizes cis variation and enables the efficient assessment of all pairs of RIL alleles relative to the tester, a much smaller study compared to the pairwise direct assessment.