Towards Unmanned Proteomics Data Generation: A Fully Automated Sample-to-Data System for Proteomic Experiments

Wang, D.; Chen, W.; Xie, L.; Xu, Y.; Huang, C.; Liu, Y.; Wang, X.; Huang, X.; Zhang, K.; Pan, M.; Wang, S.; Qin, B.; Yang, Y.; Yang, J.; Tang, L.; Tian, R.; He, F.

doi:10.1101/2025.06.02.656978

bioRxiv · 10.1101/2025.06.02.656978

Towards Unmanned Proteomics Data Generation: A Fully Automated Sample-to-Data System for Proteomic Experiments

Wang, D.·Chen, W.·Xie, L.·Xu, Y.·Huang, C.·Liu, Y.·Wang, X.·Huang, X.·Zhang, K.·Pan, M.·Wang, S.·Qin, B.·Yang, Y.·Yang, J.·Tang, L.·Tian, R.·He, F.

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Abstract

The {pi}-HuB project requires proteomic measurements of millions of low-input samples that are challenging with current technology. Here, we introduce a pilot {pi}-HuB data factory that features a fully automated sample-to-data system to enable high-throughput digitization of human samples. Our platform, as a proof-of-concept towards unmanned proteomics, was demonstrated to show longitudinal robustness and applied for spatial proteomics and the profiling of plasma and cell lines, thereby serving as the workhorse to drive {pi}-HuB.

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Wang, D., Chen, W., Xie, L., Xu, Y., Huang, C., Liu, Y., Wang, X., Huang, X., Zhang, K., Pan, M., Wang, S., Qin, B., Yang, Y., Yang, J., Tang, L., Tian, R., He, F.. 2025-06-04. Towards Unmanned Proteomics Data Generation: A Fully Automated Sample-to-Data System for Proteomic Experiments. https://doi.org/10.1101/2025.06.02.656978

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