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bioRxiv · 10.1101/2025.05.28.656662

Resistance to heavy metals and genome sequencing of ESBL-producing and non-ESBL-producing Escherichia coli

Wenyi, M.·Tianshuai, Y.·yu, D.·Yue, X.·saihui, S.·Han, S.·YiFei, Y.·hua, j.
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Abstract

The present study aimed to examine the impact of heavy metals on the resistance levels exhibited by ESBL-producing and non-ESBL-producing Escherichia coli. The minimum inhibitory concentration (MIC) of six heavy metals on two types of E. coli was determined using the broth microdilution method. Heavy metal resistance genes were detected via PCR amplification, and the gene structures were analyzed through whole genome sequencing. The findings indicated that ESBL-producing E. coli strains exhibited significantly higher levels of resistance to Cu, Zn, and Cd compared to non-ESBL-producing strains. Additionally, ESBL-producing strains harbored various plasmid-mediated heavy metal resistance genes. The results of WGS demonstrated that ESBL-producing strains carried plasmids encoding 148 heavy metal resistance genes, and the resistance to certain antibiotics (e.g., chloramphenicol) in E. coli exhibited a significantly negative correlation with resistance to specific heavy metals, including Mn and Cd (p < 0.05). The present study hypothesizes the presence of synergistic mechanisms between heavy metal resistance and antibiotic resistance in ESBL-producing E. coli, including the dual functionality of the efflux pumping system, the synergistic regulation of the pressure response system, and the physical barrier effect of biofilm formation. The experimental findings revealed the link between heavy metals and antibiotic resistance, highlighting the importance of environmental monitoring and control of heavy metal pollution. Further studies are warranted to explore the mechanisms by which heavy metals promote the transfer of genes associated with antibiotic resistance in order to formulate effective intervention strategies to combat this issue.

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Wenyi, M., Tianshuai, Y., yu, D., Yue, X., saihui, S., Han, S., YiFei, Y., hua, j.. 2025-05-28. Resistance to heavy metals and genome sequencing of ESBL-producing and non-ESBL-producing Escherichia coli. https://doi.org/10.1101/2025.05.28.656662

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