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bioRxiv · 10.1101/2025.05.27.655523

MitoTracker transfers from astrocytes to neurons independently of mitochondria

Hole, K. L.·Norkett, R.·Russell, E.·Strom, M.·Howden, J. H.·Corbett, N. J.·Brownlees, J.·Devine, M. J.
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Abstract

The neuroprotective transfer of mitochondria from astrocytes to neurons has been primarily investigated by labelling astrocytic mitochondria with the dye MitoTracker. Here we report that MitoTracker transfers to neurons from both astrocytes and astrocyte-conditioned media, independently of mitochondrial transfer. Our observations should prompt an essential re-evaluation of the literature concerning astrocyte-neuron mitochondrial transfer and in other systems in which contact-independent transfer has been observed using mitochondrial dyes.

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Hole, K. L., Norkett, R., Russell, E., Strom, M., Howden, J. H., Corbett, N. J., Brownlees, J., Devine, M. J.. 2025-05-28. MitoTracker transfers from astrocytes to neurons independently of mitochondria. https://doi.org/10.1101/2025.05.27.655523

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