Transcriptional and cellular maturation of the chick spinal cord in the context of distinct neuromuscular circuits

Abstract

The chicken spinal cord is a classic model system to study the early specification of neuronal cell types along its anterior-posterior axis. Here, we follow the ensuing maturation dynamics at limb levels with single cell resolution and contrast neuronal populations innervating appendages of distinct form and function. We use gene co-expression modules to identify rare cell populations with specific biological functions, and show that appendages with different motor outputs - wings and legs - rely on largely similar spinal cord cell type repertoires. Challenging the system with experimental alterations to the peripheral limb musculature reveals limited transcriptional changes, but spatially restricted plasticity in spinal cord motor neuron numbers. Collectively, our results provide a resource to investigate the molecular and cellular basis of neuronal maturation in the avian spinal cord and highlight the plastic nature of embryonic cells to adapt to changes in the limb periphery at both developmental and evolutionary timescales. HighlightsO_LICellular and transcriptional maturation of the developing chicken spinal cord at single-cell resolution C_LIO_LISingle-cell weighted gene co-expression network analysis isolates transcriptional maturation dynamics in different neuronal populations C_LIO_LIIn silico identification and in situ verification of novel markers for cerebrospinal fluid-contacting neurons C_LIO_LIMolecular characterization of the avian glycogen body C_LIO_LITranscriptional and compositional changes of spinal cord cell types in the context of different neuromuscular circuits C_LI