Simple, rapid, and sensitive assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil

Bogar, G.; Lennon, J. T.; Vander Stel, H. M.; Evans, S. E.

doi:10.1101/2025.05.22.654594

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bioRxiv · 10.1101/2025.05.22.654594

Simple, rapid, and sensitive assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil

Bogar, G.·Lennon, J. T.·Vander Stel, H. M.·Evans, S. E.

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Abstract

Current methods for quantifying total polysaccharides in soil are expensive or require prolonged incubation of hazardous reagents at 100{degrees}C in glass vessels. We present a fast, sensitive, and inexpensive spectrophotometric assay quantifying polysaccharides in aqueous solution, featuring an instant reaction of room-temperature reagents using disposable tubes and cuvettes.

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Bogar, G., Lennon, J. T., Vander Stel, H. M., Evans, S. E.. 2025-05-27. Simple, rapid, and sensitive assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil. https://doi.org/10.1101/2025.05.22.654594

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