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bioRxiv · 10.1101/2025.05.21.655419

A case for slow-wave morphology as a functional biomarker of gastric disease

Dowrick, J. M.·Erickson, J. C.·Du, P.·Angeli-Gordon, T. R.
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Abstract

AimUnderlying bioelectrical slow waves are critical for regulating gastric motility, and abnormal spatiotemporal slow-wave dysrhythmias are associated with a range of gastrointestinal disorders. However, the definition and role of the morphology of gastric slow-wave signals have remained limited. This study aimed to investigate the potential of gastric slow-wave morphology as an actionable biomarker. MethodsData were repurposed from a study where, following ethical approval, a control cohort (n=9) and a pathological cohort of patients with chronic unexplained nausea and vomiting (CUNV; n=8) underwent intra-operative high-resolution serosal electrical mapping (96-256 electrodes, 4.0-5.2 mm spacing). Slow waves were identified using validated software, and spatiotemporally averaged waveforms were compared between cohorts. These waveforms were replicated in a computational model of gastric slow-wave propagation to explore potential functional implications. ResultsThe slow-wave morphology of the CUNV cohort exhibited a more gradual recovery stroke compared to controls, which manifested as an increase in the normalized recovery stroke area [0.206 (95% CI: 0.169-0.247) vs. 0.134 (95% CI: 0.106-0.166); p=0.011]. Computational modeling showed that these morphological differences could drive spatial slow-wave dysrhythmias. Considering the evident functional importance of gastric slow-wave morphology, we highlighted the three typical morphological features: 1) rapid, brief upstroke, 2) downstroke, and 3) biphasic recovery stroke. ConclusionAltogether, this study presents a case for gastric slow-wave morphology as a biomarker of gastric health and disease and lays a foundation for the standardization of future slow-wave morphology research. PRACTITIONER POINTSO_LIAbnormal spatial slow-wave propagation is associated with a range of gastrointestinal motility disorders, but morphology has had limited consideration. C_LIO_LISlow-wave morphology differs between cohorts of healthy controls and patients with chronic unexplained nausea and vomiting. C_LIO_LIMultiscale mathematical modeling indicates that a disruption to the slow-wave recovery stroke may contribute to spatial disorganization. C_LI

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Dowrick, J. M., Erickson, J. C., Du, P., Angeli-Gordon, T. R.. 2025-05-26. A case for slow-wave morphology as a functional biomarker of gastric disease. https://doi.org/10.1101/2025.05.21.655419

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