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bioRxiv · 10.1101/2025.05.16.654572

cellSight: Characterizing dynamics of cells using single-cell RNA-sequencing

Chatterjee, R.·Gohel, C.·Shook, B. A.·Rahnavard, A.
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Abstract

Single-cell analysis has transformed our understanding of cellular diversity, offering insights into complex biological systems. Yet, manual data processing in single-cell studies poses challenges, including inefficiency, human error, and limited scalability. To address these issues, we propose the automated workflow cellSight, which integrates high-throughput sequencing in a user-friendly platform. By automating tasks like cell type clustering, feature extraction, and data normalization, cellSight reduces researcher workload, promoting focus on data interpretation and hypothesis generation. Its standardized analysis pipelines and quality control metrics enhance reproducibility, enabling collaboration across studies. Moreover, cellSights adaptability supports integration with emerging technologies, keeping pace with advancements in single-cell genomics. cellSight accelerates discoveries in single-cell biology, driving impactful insights and clinical translation. It is available with documentation and tutorials at https://github.com/omicsEye/cellSight.

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Chatterjee, R., Gohel, C., Shook, B. A., Rahnavard, A.. 2025-05-22. cellSight: Characterizing dynamics of cells using single-cell RNA-sequencing. https://doi.org/10.1101/2025.05.16.654572

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