Trpv1-dependent Cacna1b gene inactivation reveals cell-specific functions of CaV2.2 channels in vivo

Meir, R. Y.; Sisti, M. S.; Andrade, A.; Lipscombe, D.

doi:10.1101/2025.05.09.652131

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bioRxiv · 10.1101/2025.05.09.652131

Trpv1-dependent Cacna1b gene inactivation reveals cell-specific functions of CaV2.2 channels in vivo

Meir, R. Y.·Sisti, M. S.·Andrade, A.·Lipscombe, D.

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Abstract

Voltage-gated CaV2.2 channels underlie the N-type current, and they regulate calcium entry at many presynaptic nerve endings to control transmitter release. A role for CaV2.2 channels has been well-established in the transmission of pain information using pharmacological and global gene inactivated mouse models. However, investigation of the cell-specific actions of CaV2.2 channels would benefit from the availability of cell-restricted knockout mouse models and particularly in dissecting behavioral responses that depend on CaV2.2 channel activity. Here, we show the importance of CaV2.2 channels in Trpv1-lineage neurons in behavioral responses to sensory stimuli using Cre-dependent inactivation of the Cacna1b gene. Our work shows the cell- type specificity of CaV2.2 channels in mediating rapidly developing heat hypersensitivity and the utility of Cre-dependent inactivation of Cacna1b to discern cell-specific CaV2.2 channel functions.

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Meir, R. Y., Sisti, M. S., Andrade, A., Lipscombe, D.. 2025-05-10. Trpv1-dependent Cacna1b gene inactivation reveals cell-specific functions of CaV2.2 channels in vivo. https://doi.org/10.1101/2025.05.09.652131

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