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bioRxiv · 10.1101/2025.05.07.652656

mtgRNA-db: An annotated database of multi-target CRISPR-Cas9 guide-RNAs in the human genome

Modrek, A. S.·Huynh, K.·Do, C.·Karp, J.·Deng, Y.·Zhang, Z.·Ezhilarasan, R.·Valor, B.·Cova, G.·Skok, J. A.·Sulman, E. P.
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Abstract

CRISPR-Cas9 guide-RNA design tools can be used to identify guide-RNAs that target single human genomic loci. However, these approaches limit their effect to a single locus. Here, we generate a database of potential individual guide-RNAs that target multiple sites in the genome at once. All guide-RNAs in this database are curated with on- and off-target quantification and enrichment scores for genomic elements such as gene elements, regulatory elements, repetitive elements, and transcription factor motifs. This tool enables rationale mass targeting of genomic loci with a single guide for functional studies. We created a web-app for user-friendly guide-RNA selection (https://modreklab.shinyapps.io/guiderna/).

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Modrek, A. S., Huynh, K., Do, C., Karp, J., Deng, Y., Zhang, Z., Ezhilarasan, R., Valor, B., Cova, G., Skok, J. A., Sulman, E. P.. 2025-05-10. mtgRNA-db: An annotated database of multi-target CRISPR-Cas9 guide-RNAs in the human genome. https://doi.org/10.1101/2025.05.07.652656

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