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bioRxiv · 10.1101/2025.05.05.652170

Vitrification-warming delays preimplantation development and impairs mitochondrial function and cytoplasmic lattices integrity in mouse embryos

Abstract

Vitrification and warming of human embryos have become standardized procedures in assisted reproduction over the past two decades. Although generally considered safe, their full impact on embryo development remains unclear. Epidemiological studies have raised concerns about differences in birth weight and long-term health outcomes between newborns resulting from fresh versus frozen embryo transfers. In this study, we used mouse embryos to investigate the impact of vitrification and warming on developmental kinetics, mitochondrial function, and cytoplasmic lattice integrity. Time-lapse imaging revealed significant developmental delays across all preimplantation stages in vitrified embryos. Additionally, mitochondrial distribution, volume, and membrane potential exhibited signs of impairment. Ultrastructural analysis identified damage such as ruptured mitochondrial membranes, disrupted cytoplasmic lattices during early cell divisions, and underdeveloped mitochondrial cristae at the blastocyst stage. We hypothesize embryo vitrification and warming disrupt mitochondrial function and destabilize cytoplasmic lattice integrity, ultimately contributing to developmental delays in preimplantation embryos.

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BibTeXRIS

Barroso, M. T., Rodriguez Munoz, J. A., Sjöstrom, V., Dindini, K., Zhao, J., Rodriguez-Wallberg, K. A., Palomares, A. R.. 2025-05-06. Vitrification-warming delays preimplantation development and impairs mitochondrial function and cytoplasmic lattices integrity in mouse embryos. https://doi.org/10.1101/2025.05.05.652170

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