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bioRxiv · 10.1101/2025.05.05.652164

mRNA trafficking directs cell-size-scaling of mitochondria distribution and function

Bradbury, J. J.·Hulmes, G. E.·Viswanathan, R.·Costa, G.·Lovegrove, H. E.·Herbert, S. P.
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Abstract

The subcellular positioning of organelles is critical to their function and is dynamically adapted to changes in cell morphology. Yet, how cells sense shifts in their dimensions and redistribute organelles accordingly remains unclear. Here we reveal that cell-size-scaling of mitochondria distribution and function is directed by polarised trafficking of mRNAs. We identify a 29bp 3UTR motif in mRNA encoding TRAK2, a key determinant of mitochondria retrograde transport, that promotes cell-size-dependent targeting of TRAK2 mRNA to distal sites of cell protrusions. Cell-size-scaled mRNA polarisation in turn scales mitochondria distribution by defining the precise site of TRAK2-MIRO1 retrograde transport complex assembly. Consequently, 3UTR motif excision perturbs size-regulated transport and eradicates scaling of mitochondria positioning, triggering distal accumulation of mitochondria and progressive hypermotility as cells increase size. Together, our results reveal an RNA-driven mechanistic basis for the cell-size-scaling of organelle distribution and function that is critical to homeostatic control of motile cell behaviour.

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Bradbury, J. J., Hulmes, G. E., Viswanathan, R., Costa, G., Lovegrove, H. E., Herbert, S. P.. 2025-05-06. mRNA trafficking directs cell-size-scaling of mitochondria distribution and function. https://doi.org/10.1101/2025.05.05.652164

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