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bioRxiv · 10.1101/2025.04.28.651075

A simple, inexpensive battery-powered homeothermic warming pad for mice and rats

Fleites, I. R.·Morales, K.·Roper, S. D.
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Abstract

BackgroundAnesthesia decreases core body temperature, and this seriously compromises the physiological status of an experimental animal. Hypothermia alters many aspects of neural function. When recording nervous system activity in anesthetized animals, their core temperature must be stabilized. New methodThis report describes an inexpensive, battery-powered, temperature-controlled warming pad for mice and rats and documents its validity and utility. The device is portable, making it convenient for researchers who conduct procedures such as surgical preparations in one location and transport the anesthetized animal to another location for experimental recordings. ResultsThe device keeps anesthetized mice normothermic {+/-}0.7{degrees} for over 6 hours without supplemental warmth (e.g., heat lamp), despite >15{degrees} differential between ambient room temperature and core body temperature. We demonstrate how the warming pad can be used for in vivo imaging of neuronal activity for a prolonged period in mice. Comparison with existing methodsCommercial heating pads for small animals are expensive, somewhat bulky, and require power cords and a 120/240V source. Transporting an anesthetized animal from one location (e.g. surgical suite) to another (e.g., imaging rig) involves moving power cords. Moreover, commercial devices are not always compatible with custom stereotaxic frames, microscope stages, or holding boxes. The device described here is small, inexpensive, battery-powered, and readily adaptable to experimental set ups. ConclusionThe homeothermic heating pad provides a simple method for maintaining the core temperature of anesthetized small animals. It can be constructed in under 30 minutes, the components are readily available, and the cost is less than $100. It is exceptionally useful for experiments on mice or rats. HighlightsO_LITemperature-controlled warming pad for small rodents C_LIO_LILow cost, well under $100 C_LIO_LIComponents available from online suppliers C_LIO_LICan be assembled in less than 30 minutes C_LIO_LIDoes not require specialty tools C_LIO_LIThe device is stable and robust C_LI

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Fleites, I. R., Morales, K., Roper, S. D.. 2025-05-01. A simple, inexpensive battery-powered homeothermic warming pad for mice and rats. https://doi.org/10.1101/2025.04.28.651075

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