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bioRxiv · 10.1101/2025.04.27.647198

makeTCR: a Flexible Platform for Rapid, Scalable, Single-Step T Cell Receptor Synthesis

Hamberger, M.·Neuhoff, M.-T.·Torres, C. M.·Tan, C. L.·Boschert, T.·Errerd, A.·Lindner, J. M.·Platten, M.·Green, E. W.
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Abstract

Personalised cell therapies utilising T cell receptors (TCRs) show tremendous clinical promise, though TCR synthesis and validation techniques lag behind our ability to sequence TCR repertoires. To address this gap, we developed makeTCR: a flexible modular, scalable TCR cloning system that enables single-step, 100% fidelity assembly of {beta} and {gamma}{delta} TCRs into commonly used expression vectors. By implementing cell-free manufacturing, makeTCR enables patient-derived TCRs to be functionally validated within 48 hours. We provide an open-source, easily extensible, web-based graphical platform that integrates existing tools to simplify and standardise the manufacture of TCRs across all scales of synthesis.

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Hamberger, M., Neuhoff, M.-T., Torres, C. M., Tan, C. L., Boschert, T., Errerd, A., Lindner, J. M., Platten, M., Green, E. W.. 2025-04-28. makeTCR: a Flexible Platform for Rapid, Scalable, Single-Step T Cell Receptor Synthesis. https://doi.org/10.1101/2025.04.27.647198

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